Part:BBa_K1714000:Design
pBad/araC-RBS B0034-Ag43 thanatin as alpha domain-double terminator
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1468
Illegal BamHI site found at 1144 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1750
Illegal NgoMIV site found at 2260
Illegal NgoMIV site found at 2281
Illegal AgeI site found at 979
Illegal AgeI site found at 1413
Illegal AgeI site found at 2020
Illegal AgeI site found at 2434
Illegal AgeI site found at 2676 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
Design Notes
This part has 6 PstI sites so you shoud not digest this part by PstI.
Source
This part is derived from BBa_K759001 and it contains thanatin-Asp fragment that is synthesized by IDT.
References
Seiichi Taguchi, Kanako Kuwasako, Atsushi Suenaga, Miyuki Okada and Haruo Momose, Functional Mapping against Escherichia coli for the Broad-Spectrum Antimicrobial peptide, Thanatin, Based on an In Vivo Monitoring Assay System, J. Biochem. 2000, 128:745-754 van der Woude, Marjan W Henderson, Ian R. Regulation and function of Ag43 (flu). Annu. Rev. Microbiol. 2008, 62:153–69 Glen C. Ulett, Jaione Valle, Christophe Beloin, Orla Sherlock, Jean-Marc Ghigo and Mark A. Schembri. Functional Analysis of Antigen 43 in Uropathogenic Escherichia coli Reveals a Role in Long-Term Persistence in the Urinary Tract Infect. Immun. 2007, 75(7):3233. DOI: 10.1128/IAI.01952-06